CBQF/LAE Research Seminar “Improvement of the accuracy of analytical results in separation techniques using new methodological approaches”

23.10.2024 16:15
Escola Superior de Biotecnologia | Auditório Arménio Miranda | Edifício de Biotecnologia / Biotechnology Building

The CBQF/LAE Research Seminar “Improvement of the accuracy of analytical results in separation techniques using new methodological approaches” with Paweł Świt, from Institute of Chemistry, Faculty of Science and Technology, University of Silesia in Katowice, Poland, will take place on October 23, at 4:15 pm, in Arménio Miranda Auditórium, in the Biotechnology Building.

Abstract
Nowadays, due to the increasing demand from the economic environment, many chemical analyses are performed in analytical laboratories. For this reason, it seems reasonable to use such modifications in the course of the analytical procedure that will enable effective checking of the occurrence of systematic errors in the tested analytical system, elimination of these errors, and obtaining the most accurate analytical result possible together with the assessment of this accuracy. In recent years, several new calibration approaches have been developed that allow for the verification of accuracy. One of the calibration approaches included in this group is the integrated calibration method (ICM) in the basic version and in the version of the complementary dilution method (CDM), combining the features of both external calibration (EC) and the standard addition method (SAM) in a single procedure. The most important advantages of this strategy are simplicity and speed of execution, as well as versatility and ease of interpretation of the obtained data. Another approach - the H-point standard addition method (HPSAM) allows for the elimination of additive interferences. This lecture will present studies in which the above calibration methods were verified, modified, and combined into innovative calibration approaches. These methods have been verified in separation techniques by using high-performance liquid chromatography with different detection in three different areas: biomedical, environmental, and food research. In the first area, the quality of analytical results was improved in the simultaneous determination of glutamate and aspartate, as well as in acetylcholine in cerebrospinal fluid samples collected from laboratory animals after testing new psychoactive substances. Environmental studies focused on the determination of polycyclic aromatic hydrocarbons (PAHs) in the context of simulated fires and in order to more reliably assess the sources of these contaminants. Instead, in the food area, the focus was on the determination of bisphenols in store bills and canned food with the use of two different methodological approaches. Research on the development of new, innovative calibration methods provides an opportunity to effectively investigate and eliminate interference effects and consequently obtain results with increased accuracy.

Biographical notes
Paweł Świt, Ph.D., Eng. – A graduate of the Faculty of Chemistry, Jagiellonian University in Krakow, and the Faculty of Materials Science and Ceramics, AGH University of Science and Technology in Krakow. In 2017, he defended a Ph.D. dissertation entitled ‘Assessment and elimination of interference effects using innovative calibration methods’. Afterward, he started working at the Maj Institute of Pharmacology, Polish Academy of Sciences, where he was involved in the determination of neurotransmitters levels in brain dialysates after the administration of new psychoactive substances using high-performance liquid chromatography (HPLC). In 2019, he joined the Jagiellonian Centre for Experimental Therapeutics, and his research interests were related to surface-enhanced Raman spectroscopy (SERS). Currently, he works at the Institute of Chemistry of the University of Silesia. His scientific interests includes separation techniques, chromatography, development of analytical methods, validation of analytical methods, analytical and bioanalytical environmental chemistry, flow analysis and chemistry, and the use of chemistry in conservation.